Anti-HSF1 Polyclonal Antibody
Cat: K010070P
Summary:
| 【Product name】：Anti-HSF1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：3297 | 【Swiss Prot】：Q00613 |
| 【Observed】：83kDa | |
| 【Tested applications】：IHC,WB,IP,ELISA,IF | |
| 【Recommended dilution】：WB 1:1000-4000. IHC 1:500-2000. IF 1:20-200. | |
| 【Immunogen】：Recombinant protein of human HSF1 | |
| 【Public Immunogen Range】：230-529aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
HSF1 belongs to heat-shock transcription factor that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishs a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa that likely represent different phosphorylation states (PMID: 18434628).
Verified picture:
Western blot analysis with HSF1 antibody diluted at 1:2000;Lane: HEK293 HEK293T HeLa K-562.
Immunohistochemistry of paraffin-embedded human lung cancer with HSF1 antibody diluted at 1:100.
Immunofluorescence analysis of A431 using HSF1 antibody diluted at 1:50.