Anti-EIF5B Polyclonal Antibody
Cat: K112761P
Summary:
| 【Product name】：Anti-EIF5B Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Octanoic acid-ammonium sulfate precipitation |
| 【Gene ID】：9669 | 【Swiss Prot】：O60841 |
| 【Observed】：179kDa | |
| 【Tested applications】：WB | |
| 【Recommended dilution】：WB 1:1000-3000. | |
| 【Immunogen】：A synthetic peptide of Human EIF5B | |
| 【Public Immunogen Range】：750-800/1220aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Verified picture:
Western blot analysis with EIF5B antibody diluted at 1:2000;Lane: K-562,HepG2.