Anti-Phospho-PERK (Thr982) Polyclonal Antibody
Cat: K010157P
Summary:
| 【Product name】：Anti-Phospho-PERK (Thr982) Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：9451 | 【Swiss Prot】：Q9NZJ5 |
| 【Observed】：130kDa | |
| 【Tested applications】：ELISA,WB,IF,ICC,IHC | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ELISA 1:10000. | |
| 【Immunogen】：A synthetic peptide of human Phospho-PERK (Thr982) | |
| 【Public Immunogen Range】：947-996aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Verified picture:
Western blot analysis with Phospho-PERK (Thr982) antibody diluted at 1:1000;Lane: A453 AD293 HeLa.
Immunohistochemistry of paraffin-embedded mouse kidney with Phospho-PERK (Thr982) antibody diluted at 1:100.