Anti-ATG14 Polyclonal Antibody
Cat: K113576P
Summary:
| 【Product name】：Anti-ATG14 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：22863 | 【Swiss Prot】：Q6ZNE5 |
| 【Observed】：55kDa | |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human ATG14 | |
| 【Public Immunogen Range】：1-344aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity.
Verified picture:
Western blot analysis with ATG14 antibody diluted at 1:2000;Lane: Mouse brain,Rat brain.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with ATG14 antibody diluted at 1:100.