Anti-PCNA Monoclonal Antibody
Cat: K000119M
Summary:
| 【Product name】：Anti-PCNA Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG2b | 【Purification】：Fractional precipitation |
| 【Gene ID】：5111 | 【Swiss Prot】：P12004 |
| 【Tested applications】：ICC,IHC,IF,IP,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-200. IF 1:100-200. IP 1:10-20. ICC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human PCNA | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.[provided by RefSeq,Jul 2008]
Verified picture:
Western blot analysis with PCNA antibody diluted at 1:1000;Lane: CHO-K1 C6 COS7 3T3 HeLa.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with PCNA antibody diluted at 1:400
Immunofluorescence analysis of HeLa using PCNA antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using PCNA antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.