Anti-GSK3B Monoclonal Antibody
Cat: K000187M
Summary:
| 【Product name】：Anti-GSK3B Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Saturated ammonium sulfate precipitation |
| 【Gene ID】：2932 | 【Swiss Prot】：P49841 |
| 【Tested applications】：IHC,WB | |
| 【Recommended dilution】：WB 1:1000-2000. IHC 1:100-200. | |
| 【Immunogen】：Synthetic Peptide | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
Verified picture:
Western blot analysis with GSK3�� antibody diluted at 1:1000, lane1-3: Hela,3T3 Cell, Rat brain tissue
Immunohistochemistry of paraffin-embedded Human Breast Carcinoma with GSK3B antibody diluted at 1:200.