Anti-Cytokeratin 18 Monoclonal Antibody
Cat: K000209M
Summary:
| 【Product name】：Anti-Cytokeratin 18 Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Saturated ammonium sulfate precipitation |
| 【Gene ID】：3875 | 【Swiss Prot】：P05783 |
| 【Tested applications】：IHC,IF,WB | |
| 【Recommended dilution】：WB 1:1000-3000. IF 1:100-200. IHC 1:200-500. | |
| 【Immunogen】：Recombinant Protein | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Verified picture:
Western blot analysis with Cytokeratin 18 antibody diluted at 1:2000;Lane: HepG2,HeLa,Mouse liver,C2C12,Rat heart,Mouse skeletal muscle.
Immunohistochemistry of paraffin-embedded Human breast cancer with Cytokeratin 18 antibody diluted at 1:500
Immunofluorescence analysis of HeLa using Cytokeratin 18 antibody diluted at 1:100.