Anti-eIF4A1 Monoclonal Antibody
Cat: K000213M
Summary:
| 【Product name】：Anti-eIF4A1 Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Saturated ammonium sulfate precipitation |
| 【Gene ID】：1973 | 【Swiss Prot】：P60842 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:1000-3000. IF 1:100-200. IHC 1:100-200. | |
| 【Immunogen】：Synthetic Peptide | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.
Verified picture:
Western blot analysis with eIF4A1 antibody diluted at 1:2000 ,lane1-5: 293T, Hela, HepG2, Mouse Brain tissue, Rat Brain tissue
Immunohistochemistry of paraffin-embedded Human Lung cancer with eIF4A1 antibody diluted at 1:200
Immunofluorescence analysis of HeLa using eIF4A1 antibody diluted at 1:100.