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Anti-RAF1 Polyclonal Antibody
Cat: K000318P
Summary:
【Product name】:Anti-RAF1 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:5894 【Swiss Prot】:P04049
【Tested applications】:IHC,WB,IF
【Recommended dilution】:WB 1:500-1000. IHC 1:50-200. IF 1:50-200.
【Immunogen】:Recombinant protein of human RAF1
【Public Immunogen Range】:190-350aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Verified picture:
Immunohistochemistry of paraffin-embedded rat liver tissue using RAF1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat heart tissue using RAF1 antibody at dilution of 1:100 (x40 lens)
Western blot analysis with RAF1 antibody diluted at 1:1000;Lane: Mouse liver.
Immunohistochemistry of paraffin-embedded human colon carcinoma with RAF1 antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using RAF1 antibody diluted at 1:100.