Anti-EIF4E Polyclonal Antibody
Cat: K000364P
Summary:
| 【Product name】：Anti-EIF4E Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity Purification |
| 【Gene ID】：1977 | 【Swiss Prot】：P06730 |
| 【Tested applications】：WB,IHC,IP,IF | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-100. IF 1:50-200. IP 0.5μg-4μg. | |
| 【Immunogen】：A synthetic peptide of human EIF4E | |
| 【Public Immunogen Range】：1-100aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Verified picture:
Immunohistochemistry of paraffin-embedded Breast cancer(hu) using EIF4 antibody at dilution of 1:100(x100 lens x200 lens).
Western blot analysis with EIF4E antibody diluted at 1:1000;Lane: HeLa 293T HepG2 NIH3T3 Mouse testis Rat testis.
Immunohistochemistry of paraffin-embedded human lung cancer with EIF4E antibody diluted at 1:75.
Immunofluorescence analysis of U2OS cells using EIF4E antibody diluted at 1:100.