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Anti-DsbA-Tag Monoclonal Antibody
Cat: K001375M
Summary:
【Product name】:Anti-DsbA-Tag Monoclonal Antibody 【Source】:Mouse
【Isotype】:IgG2b 【Purification】:Affinity purification
【Gene ID】:8114936 【Swiss Prot】:P0AEG4
【Tested applications】:WB
【Recommended dilution】:WB 1:1000-10000.
【Immunogen】:Recombinant protein of DsbA
【Storage】:Store at -20℃. Avoid freeze / thaw cycles.
Background:
Disulphide oxidoreductase (DsbA) is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. It is a member of the thioredoxin superfamily. DsbA introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.
Verified picture:
Western blot analysis with DsbA-Tag antibody diluted at 1:5000;Lane: Dsba recombinant protein.