Anti-H2AFX(Phospho-Ser139) Monoclonal Antibody
Cat: K001451M
Summary:
| 【Product name】：Anti-H2AFX(Phospho-Ser139) Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG2a | 【Purification】：Affinity purification |
| 【Gene ID】：3014 | 【Swiss Prot】：P16104 |
| 【Tested applications】：ICC,IF,WB | |
| 【Recommended dilution】：WB 1:500-1000. IF 1:50-200. ICC 1:50-200. | |
| 【Immunogen】：A synthetic peptide of Human H2AFX(Phospho-Ser139) | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Verified picture:
Western blot analysis with H2AFX(Phospho-Ser139) antibody diluted at 1:1000;Lane: 3T3 Hydroxyurea treated 3T3.
Immunofluorescence analysis of Hydroxyureatreated HeLa using H2AFX(Phospho-Ser139) antibody diluted at 1:100.