Anti-BAK1 Polyclonal Antibody
Cat: K001591P
Summary:
| 【Product name】：Anti-BAK1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：578 | 【Swiss Prot】：Q16611 |
| 【Tested applications】：IP,IF,WB | |
| 【Recommended dilution】：WB 1:500-1000. IF 1:50-200. IP 0.5μg-4μg. | |
| 【Immunogen】：A synthetic peptide of human BAK1 | |
| 【Public Immunogen Range】：50-150aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Verified picture:
Immunofluorescence analysis of HeLa cell using Bak1 antibody. Blue: DAPI for nuclear staining.
Western blot analysis with BAK1 antibody diluted at 1:1000;Lane: THP-1.
Immunofluorescence analysis of U2OS cells using BAK1 antibody diluted at 1:100.