Anti-NEFL Polyclonal Antibody
Cat: K001635P
Summary:
| 【Product name】：Anti-NEFL Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：4747 | 【Swiss Prot】：P07196 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-200. IF 1:50-200. | |
| 【Immunogen】：Recombinant Protein of human NEFL | |
| 【Public Immunogen Range】：60-250aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Verified picture:
Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibody at dilution of 1:200 (x400 lens).
Immunofluorescence analysis of HeLa cell using NEFL antibody. Blue: DAPI for nuclear staining.
Western blot analysis with NEFL antibody diluted at 1:1000;Lane: 293T SH-SY5Y U-251MG Mouse brain Rat brain.
Immunofluorescence analysis of rat brain using NEFL antibody diluted at 1:100.