Anti-PARP Polyclonal Antibody
Cat: K001766P
Summary:
| 【Product name】：Anti-PARP Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：142 | 【Swiss Prot】：P09874 |
| 【Tested applications】：WB,IF | |
| 【Recommended dilution】：WB 1:1000-5000. IF 1:50-200. | |
| 【Immunogen】：A synthetic peptide of human PARP | |
| 【Public Immunogen Range】：81-390aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Verified picture:
Immunohistochemistry of paraffin-embedded human esophageal cancer using PARP1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U2OS cells using PARP1 antibody (A0942) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis with PARP antibody diluted at 1:2000;Lane: HeLa Jurkat A549.