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Anti-MRPL32 Polyclonal Antibody
Cat: K001805P
Summary:
【Product name】:Anti-MRPL32 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:64983 【Swiss Prot】:Q9BYC8
【Tested applications】:IF,WB
【Recommended dilution】:WB 1:500-2000.IF 1:50-200.
【Immunogen】:Recombinant protein of human MRPL32
【Public Immunogen Range】:19-188aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.
Verified picture:
Immunohistochemistry of paraffin-embedded Breast cancer(hu) using MRPL32 antibody at dilution of 1:100 (x100 lens��x200 lens).
Western blot analysis with MRPL32 antibody diluted at 1:1000;Lane: Jurkat Raji MCF7 HepG2 A549 Mouse heart Mouse kidney Mouse liver.
Immunofluorescence analysis of U2OS cells using MRPL32 antibody diluted at 1:100.