Anti-PHGDH Polyclonal Antibody
Cat: K002010P
Summary:
| 【Product name】：Anti-PHGDH Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：26227 | 【Swiss Prot】：O43175 |
| 【Tested applications】：WB,IP,IF | |
| 【Recommended dilution】：WB 1:500-1000. IF 1:100-500. IP 0.5μg-4μg. | |
| 【Immunogen】：Recombinant protein of human PHGDH | |
| 【Public Immunogen Range】：264-533aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
Verified picture:
Western blot analysis with PHGDH antibody diluted at 1:1000;Lane: HeLa HT-29 MCF7 NIH3T3 C6 Mouse brain.
Immunofluorescence analysis of HeLa cells using PHGDH antibody diluted at 1:100.