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Anti-H2AFX Polyclonal Antibody
Cat: K002129P
Summary:
【Product name】:Anti-H2AFX Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:3014 【Swiss Prot】:P16104
【Tested applications】:WB,IHC,IF
【Recommended dilution】:WB 1:500-1000.IHC 1:50-200.IF 1:50-200.
【Immunogen】:A synthetic peptide of human H2AFX
【Public Immunogen Range】:50-143aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Verified picture:
Immunohistochemistry of paraffin-embedded Stomach cancer(hu) using H2AFX antibody at dilution of 1:100 (x100 lens��x200 lens).
Western blot analysis with H2AFX antibody diluted at 1:700;Lane: 293T NIH3T3 C2C12 Jurkat Raji Rat thymus.
Immunohistochemistry of paraffin-embedded rat testis with H2AFX antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using H2AFX antibody diluted at 1:100.