Anti-CDC25A Polyclonal Antibody
Cat: K002353P
Summary:
| 【Product name】：Anti-CDC25A Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：993 | 【Swiss Prot】：P30304 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-200. IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human CDC25A | |
| 【Public Immunogen Range】：86-328aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Verified picture:
Immunohistochemistry of paraffin-embedded rat brain using CDC25A antibody at dilution of 1:200.
Western blot analysis with CDC25A antibody diluted at 1:1000;Lane: HeLa Raji Mouse liver Mouse testis Rat liver Rat testis.
Immunohistochemistry of paraffin-embedded rat liver with CDC25A antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using CDC25A antibody diluted at 1:100.