Anti-DHFR Polyclonal Antibody
Cat: K002868P
Summary:
| 【Product name】：Anti-DHFR Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：1719 | 【Swiss Prot】：P00374 |
| 【Tested applications】：WB,IF | |
| 【Recommended dilution】：WB 1:500-2000. IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human DHFR | |
| 【Public Immunogen Range】：1-187aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
Verified picture:
Western blot analysis with DHFR antibody diluted at 1:1000;Lane: HeLa 22Rv1 K-562 HL-60 THP-1 Raji Mouse liver.
Immunofluorescence analysis of A549 cells using DHFR antibody diluted at 1:100.