Anti-POLR2A Polyclonal Antibody
Cat: K003221P
Summary:
| 【Product name】：Anti-POLR2A Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5430 | 【Swiss Prot】：P24928 |
| 【Tested applications】：IF,IP,WB | |
| 【Recommended dilution】：WB 1:500-2000. IF 1:50-200. IP 0.5μg-4μg. | |
| 【Immunogen】：Recombinant protein of human POLR2A | |
| 【Public Immunogen Range】：1-260aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Verified picture:
Western blot analysis with POLR2A antibody diluted at 1:1000;Lane: HeLa Jurkat.
Immunofluorescence analysis of H9C2 cells using POLR2A antibody diluted at 1:100.