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Anti-DiMethyl-Histone H3-K9 Polyclonal Antibody
Cat: K003330P
Summary:
【Product name】:Anti-DiMethyl-Histone H3-K9 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:8290 【Swiss Prot】:Q16695
【Tested applications】:IHC,WB,ChIP-seq,ChIP,IP,IF,DB
【Recommended dilution】:DB 1:500-2000. WB 1:500-1000. IHC 1:50-200. IF 1:50-200. ChIP 5μg. ChIP-seq 1:50-200.
【Immunogen】:A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
【Public Immunogen Range】:1-100aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Verified picture:
Immunohistochemistry of paraffin-embedded rat liver tissue using DiMethyl-Histone H3-K9 antibody at dilution of 1:200 (x40 lens).
Western blot analysis with DiMethyl-Histone H3-K9 antibody diluted at 1:1000;Lane: HeLa NIH3T3 C6.
Immunohistochemistry of paraffin-embedded rat heart with DiMethyl-Histone H3-K9 antibody diluted at 1:100.
Immunofluorescence analysis of A549 cells using DiMethyl-Histone H3-K9 antibody diluted at 1:100.