Anti-TriMethyl-Histone H3-K27 Polyclonal Antibody
Cat: K003334P
Summary:
| 【Product name】：Anti-TriMethyl-Histone H3-K27 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：8290 | 【Swiss Prot】：Q16695 |
| 【Tested applications】：IF,IHC,WB,DB,ChIP-seq,ChIP,IP | |
| 【Recommended dilution】：DB 1:500-1000. WB 1:500-1000. IHC 1:50-200. IF 1:50-200. IP 0.5ug-4ug. ChIP 5μg. ChIP-seq 1:20-100. | |
| 【Immunogen】：A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 | |
| 【Public Immunogen Range】：1-100aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Verified picture:
Western blot analysis with TriMethyl-Histone H3-K27 antibody diluted at 1:1000;Lane: MCF7 acid extract 293T acid extract C2C12 acid extract NIH3T3 acid extract C6 acid extract H3 protein.
Immunohistochemistry of paraffin-embedded rat testis tissue using TriMethyl-Histone H3-K27 antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded rat ovary with TriMethyl-Histone H3-K27 antibody diluted at 1:100.
Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K27 antibody diluted at 1:100.