Anti-RFC4 Polyclonal Antibody
Cat: K004030P
Summary:
| 【Product name】：Anti-RFC4 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5984 | 【Swiss Prot】：P35249 |
| 【Tested applications】：IP,IF,WB | |
| 【Recommended dilution】：WB 1:500-2000. IF 1:50-100. IP 0.5μg-4μg. | |
| 【Immunogen】：Recombinant protein of human RFC4 | |
| 【Public Immunogen Range】：204-363aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Verified picture:
Western blot analysis with RFC4 antibody diluted at 1:1000;Lane: HeLa SW480 22Rv1 SW620 K-562 HepG2 MCF7.
Immunofluorescence analysis of U20S cell using RFC4 antibody. Blue: DAPI for nuclear staining.