Anti-GOT1 Polyclonal Antibody
Cat: K004233P
Summary:
| 【Product name】：Anti-GOT1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：2805 | 【Swiss Prot】：P17174 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-200. IF 1:10-100. | |
| 【Immunogen】：Recombinant protein of human GOT1 | |
| 【Public Immunogen Range】：244-413aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Verified picture:
Immunohistochemistry of paraffin-embedded Esophageal cancer(hu) using GOT1 antibody at dilution of 1:100 (x100 lens,x200 lens).
Western blot analysis with GOT1 antibody diluted at 1:1000;Lane: HepG2 Mouse brain Mouse liver Mouse heart Rat brain Rat liver.
Immunohistochemistry of paraffin-embedded rat heart with GOT1 antibody diluted at 1:100.
Immunofluorescence analysis of U2OS cells using GOT1 antibody diluted at 1:50.