Anti-SF3B2 Polyclonal Antibody
Cat: K004274P
Summary:
| 【Product name】：Anti-SF3B2 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：10992 | 【Swiss Prot】：Q13435 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-2000. IHC 1:50-200. IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human SF3B2 | |
| 【Public Immunogen Range】：616-895aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Verified picture:
Western blot analysis with SF3B2 antibody diluted at 1:1000;Lane: HeLa MCF7 SW620 A375 Mouse testis.
Immunohistochemistry of paraffin-embedded human oophoroma using SF3B2 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of MCF7 cell using SF3B2 antibody.
Immunohistochemistry of paraffin-embedded rat heart with SF3B2 antibody diluted at 1:100.
Immunofluorescence analysis of A549 cells using SF3B2 antibody diluted at 1:100.