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Anti-NOTCH1 Polyclonal Antibody
Cat: K005358P
Summary:
【Product name】:Anti-NOTCH1 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:4851 【Swiss Prot】:P46531
【Tested applications】:IF,IHC,WB
【Recommended dilution】:WB 1:500-1000.IHC 1:50-200.IF 1:50-200.
【Immunogen】:Recombinant protein of human NOTCH1
【Public Immunogen Range】:2456-2555aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Verified picture:
Western blot analysis with NOTCH1 antibody diluted at 1:1000;Lane: Rat brain.
Immunohistochemistry of paraffin-embedded rat spleen using NOTCH1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat ovary with NOTCH1 antibody diluted at 1:150.
Immunofluorescence analysis of HeLa cells using NOTCH1 antibody diluted at 1:100.