Anti-CHEK1 Polyclonal Antibody
Cat: K005372P
Summary:
| 【Product name】：Anti-CHEK1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：1111 | 【Swiss Prot】：O14757 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000.IHC 1:50-200.IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human CHEK1 | |
| 【Public Immunogen Range】：148-307aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Verified picture:
Western blot analysis with CHEK1 antibody diluted at 1:1000;Lane: NIH3T3 Mouse testis Rat testis.
Immunohistochemistry of paraffin-embedded human kidney cancer using CHEK1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded Mouse spleen with CHEK1 antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using CHEK1 antibody diluted at 1:100.