Anti-SHC1 Polyclonal Antibody
Cat: K005417P
Summary:
| 【Product name】：Anti-SHC1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：6464 | 【Swiss Prot】：P29353 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000.IHC 1:50-200.IF 1:50-200. | |
| 【Immunogen】：A synthetic peptide of human SHC1 | |
| 【Public Immunogen Range】：300-400aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
Verified picture:
Western blot analysis with SHC1 antibody diluted at 1:1000;Lane: Jurkat cells.
Immunohistochemistry of paraffin-embedded rat kidney using SHC1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma with SHC1 antibody diluted at 1:100.
Immunofluorescence analysis of A549 cells using SHC1 antibody diluted at 1:100.