Anti-POU5F1 Polyclonal Antibody
Cat: K005554P
Summary:
| 【Product name】：Anti-POU5F1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5460 | 【Swiss Prot】：Q01860 |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:500-1000.IHC 1:50-200. | |
| 【Immunogen】：A synthetic peptide of human POU5F1 | |
| 【Public Immunogen Range】：210-310aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Verified picture:
Western blot analysis with POU5F1 antibody diluted at 1:1000;Lane: Mouse embryo.
Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse fetal Brain with POU5F1 antibody diluted at 1:100.