Anti-SMAD4 Polyclonal Antibody
Cat: K006651P
Summary:
| 【Product name】：Anti-SMAD4 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：4089 | 【Swiss Prot】：Q13485 |
| 【Tested applications】：IHC,IP,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:10-100. IP 1:200. | |
| 【Immunogen】：Recombinant protein of human SMAD4 | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE).
Verified picture:
Western blot analysis with SMAD4antibody diluted at 1:500; Lane: SKOV3,HEPG2
Immunohistochemistry of paraffin-embedded Human stomach cancer tissue with SMAD4 antibody diluted at 1:20
Immunoprecipitation analysis of Hela extracts using SMAD4 antibody(K006651P,IP:1:200;WB:1:1000) and SMAD3 antibody(K000497P,IP:1:200).Control IgG: Normal Rabbit IgG.