Anti-PARP Monoclonal Antibody
Cat: K200026M
Summary:
| 【Product name】：Anti-PARP Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Affinity purification |
| 【Gene ID】：142 | 【Swiss Prot】：P09874 |
| 【Tested applications】：WB;IHC | |
| 【Recommended dilution】：WB 1:2000-4000. IHC 1:50-200. | |
| 【Immunogen】：A synthetic peptide coupled to KLH | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.
Verified picture:
Immunohistochemistry of paraffin-embedded Human stomach cancer with PARP antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded Human prostate cancer with PARP antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded Human ovarian cancer with PARP antibody diluted at 1:200
Western blot analysis with PARP antibody diluted at 1:3000;Lane: L-02