Anti-PCNA Monoclonal Antibody
Cat: K200030M
Summary:
| 【Product name】：Anti-PCNA Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Affinity purification |
| 【Gene ID】：5111 | 【Swiss Prot】：P12004 |
| 【Tested applications】：IHC;WB | |
| 【Recommended dilution】：WB 1:2000-5000. IHC 1:50-300. | |
| 【Immunogen】：A synthetic peptide coupled to KLH | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Verified picture:
Immunohistochemistry of paraffin-embedded Human ovarian cancer with PCNA antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded Human cervical cancer with PCNA antibody diluted at 1:200
Western blot analysis with PCNA antibody diluted at 1:3000;Lane: Hela