Anti-Caspase 9/P35/P10 Monoclonal Antibody
Cat: K009429M
Summary:
| 【Product name】：Anti-Caspase 9/P35/P10 Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG2b | 【Purification】：Affinity purification |
| 【Gene ID】：842 | 【Swiss Prot】：P55211 |
| 【Tested applications】：IHC,ELISA,IP,WB,IF | |
| 【Recommended dilution】：WB 1:500-2000. IP 0.5-4.0 ug for IP and 1:500-2000 for WB. IHC 1:100-400. IF 1:50-500. | |
| 【Immunogen】：Recombinant protein of human Caspase 9/p35/p10 | |
| 【Public Immunogen Range】：141-392aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Verified picture:
Western blot analysis with Caspase 9/P35/P10 antibody diluted at 1:5000;Lane: Jurkat
Immunohistochemistry of paraffin-embedded Human lymphoma with Caspase 9/P35/P10 antibody diluted at 1:200
Immunofluorescence analysis of HeLa cells using Caspase 9/p35/p10 antibody diluted at 1:100.