Anti-Villin-1 Monoclonal Antibody
Cat: K009443M
Summary:
| 【Product name】：Anti-Villin-1 Monoclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：7429 | 【Swiss Prot】：P09327 |
| 【Tested applications】：IF,ICC,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:20-200. ICC 1:100-500. IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human Villin-1 | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Villin can cap, nucleate, sever and bundle actin in a calcium and phosphoinositide regulated manner. It is associated with the microvillar actin core bundle of intestinal and renal brush border implicated in adsorption. Villin is composed of six repeats, each containing 150 residues that together constitute the core domain followed by the carboxyl terminal headpiece domain of 87 residues. The core domain retains the calcium dependent capping nucleating and severing activity, whereas the headpiece domain contributes towards actin filament bundling and binding F actin, independently of Calcium.
Verified picture:
Western blot analysis with villin antibody diluted at 1:1000;Lane: Mouse Colon tissue Mouse Kidney tissue Rat Kidney tissue.
Immunohistochemistry of paraffin-embedded Rat colon with villin antibody diluted at 1:200.