Anti-phospho-IKB alpha (Ser32 + Ser36) Polyclonal Antibody
Cat: K009559P
Summary:
| 【Product name】：Anti-phospho-IKB alpha (Ser32 + Ser36) Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：4792 | 【Swiss Prot】：P25963 |
| 【Tested applications】：ELISA,IF,ICC,IHC,WB | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. ICC 1:100. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human IKB alpha around the phosphorylation site of Ser32/36 | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Verified picture:
Western blot analysis with phospho-IKB alpha (Ser32 + Ser36) antibody diluted at 1:1000;Lane: HepG2 Hela Siha U251.
Immunohistochemistry of paraffin-embedded Rat lung tissue using phospho-IKB alpha (Ser32 + Ser36) antibody at dilution of 1:200 .
Immunohistochemistry of paraffin-embedded Rat lung with phospho-IKB alpha (Ser32 + Ser36) antibody diluted at 1:200.
Immunofluorescence analysis of HepG2 cell using phospho-IKB alpha (Ser32 + Ser36) antibody diluted at 1:100.