Anti-RB1 Polyclonal Antibody
Cat: K009579P
Summary:
| 【Product name】：Anti-RB1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5925 | 【Swiss Prot】：P06400 |
| 【Tested applications】：ELISA,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human RB1 | |
| 【Public Immunogen Range】：51-150/928aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx,
Verified picture:
Western blot analysis with Rb antibody diluted at 1:300;Lane: HL60.
Immunohistochemistry of paraffin-embedded Rat brain with RB1 antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded rat brain tissue with Rb antibody diluted at 1:200.