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Anti-TUBB3 Polyclonal Antibody
Cat: K108970P
Summary:
【Product name】:Anti-TUBB3 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:10381 【Swiss Prot】:Q13509
【Tested applications】:WB;IHC;IF,IHC,WB,ELISA,IF
【Recommended dilution】:WB 1:8000-12000. IHC 1:25-100. IF 1:50-200.
【Immunogen】:A synthetic peptide of human TUBB3
【Public Immunogen Range】:5-55/450aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Verified picture:
Western blot analysis with TUBB3 antibody diluted at 1:10000;Lane: SH-SY5Y,A549,HepG2,PC-3M-IE8,Hela,MCF7,MDA-MB-231,NIH3T3,Human breast cancer,Mouse brain,Mouse testis,Rat brain.
Immunohistochemistry of paraffin-embedded Human breast cancer with TUBB3 antibody diluted at 1:50.
Immunofluorescence analysis of NIH3T3 cells using TUBB3 antibody diluted at 1:100.