下载
Anti-Mki67 Polyclonal Antibody
Cat: K009725P
Summary:
【Product name】:Anti-Mki67 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:17345 【Swiss Prot】:E9PVX6
【Tested applications】:IHC,IF
【Recommended dilution】:IHC 1:500-1200. IF 1:500-1200.
【Immunogen】:A synthetic peptide of mouse Ki67
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Verified picture:
Immunohistochemistry of paraffin-embedded rat colon with Mki67 antibody diluted at 1:800.
Immunofluorescence analysis of mouse testis using Mki67 antibody diluted at 1:600.