Anti-HLA-DOB Polyclonal Antibody
Cat: K109024P
Summary:
| 【Product name】：Anti-HLA-DOB Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：3112 | 【Swiss Prot】：P13765 |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human HLA-DOB | |
| 【Public Immunogen Range】：1-273aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Verified picture:
Immunohistochemistry of paraffin-embedded Human colorectal cancer with HLA-DOB antibody diluted at 1:100
Western blot analysis with HLA-DOB antibody diluted at 1:2000;Lane: L-02