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Anti-Histone H3 Monoclonal Antibody
Cat: K200065M
Summary:
【Product name】:Anti-Histone H3 Monoclonal Antibody 【Source】:Mouse
【Isotype】:IgG1 【Purification】:Affinity purification
【Gene ID】:8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 【Swiss Prot】:P68431
【Tested applications】:WB,IF,IP,IHC
【Recommended dilution】:WB 1:1000-3000. IHC 1:100-300. IF 1:100-300.
【Immunogen】:Recombinant Protein
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Verified picture:
Western blot analysis with Histone H3 antibody diluted at 1:2000;Lane: HeLa,Raw264.7,Mouse brain,Rat brain.
Immunohistochemistry of paraffin-embedded Human lung cancer with Histone H3 antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with Histone H3 antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human breast cancer with Histone H3 antibody diluted at 1:200.
Immunofluorescence analysis of Hela cells using Histone H3 antibody diluted at 1:200.