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Anti-RPL14 Polyclonal Antibody
Cat: K109132P
Summary:
【Product name】:Anti-RPL14 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Octanoic acid-ammonium sulfate precipitation
【Gene ID】:9045 【Swiss Prot】:P50914
【Tested applications】:WB,IF,IHC
【Recommended dilution】:WB 1:2000-5000. IHC 1:50-200. IF 1:50-200.
【Immunogen】:Recombinant protein of human RPL14
【Public Immunogen Range】:1-215aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Verified picture:
Immunohistochemistry of paraffin-embedded Human pancreatic cancer with RPL14 antibody diluted at 1:100
Western blot analysis with RPL14 antibody diluted at 1:3000;Lane: HeLa,293T,Jurkat,MCF7,HepG2
Immunofluorescence analysis of HepG2 cells using RPL14 antibody diluted at 1:100