Anti-phospho-PI3KCA (Tyr317) Polyclonal Antibody
Cat: K009756P
Summary:
| 【Product name】：Anti-phospho-PI3KCA (Tyr317) Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5290 | 【Swiss Prot】：P42336 |
| 【Tested applications】：ELISA,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human PI3KCA around the phosphorylation site of Tyr317 | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.
Verified picture:
Western blot analysis with phospho-PI3KCA (Tyr317) antibody diluted at 1:1000;Lane: NIH/3T3 Raw264.7 Jurkat MCF-7 A431 Siha.
Immunohistochemistry of paraffin-embedded Mouse brain with phospho-PI3KCA (Tyr317) antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded mouse brain with phospho-PI3KCA (Tyr317) antibody diluted at 1:200.