Anti-ATG5 Polyclonal Antibody
Cat: K000317P
Summary:
| 【Product name】：Anti-ATG5 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：9474 | 【Swiss Prot】：Q9H1Y0 |
| 【Tested applications】：IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-200. IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human ATG5 | |
| 【Public Immunogen Range】：1-275aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Verified picture:
Western blot analysis with ATG5 antibody diluted at 1:1000;Lane: HepG2 NIH3T3 K-562 C6 SH-SY5Y.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with ATG5 antibody diluted at 1:100
Immunohistochemistry of paraffin-embedded Mouse kidney with ATG5 antibody diluted at 1:100.
Immunofluorescence analysis of K-562 cells using ATG5 antibody diluted at 1:100.