Anti-FTO Polyclonal Antibody
Cat: K110456P
Summary:
| 【Product name】：Anti-FTO Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：79068 | 【Swiss Prot】：Q9C0B1 |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：A synthetic peptide of human FTO | |
| 【Public Immunogen Range】：190-240/505aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed:22002720, PubMed:26458103, PubMed:28002401, PubMed:30197295, PubMed:26457839, PubMed:25452335). Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103, PubMed:30197295, PubMed:26457839, PubMed:25452335). M6A demethylation by FTO affects mRNA expression and stability (PubMed:30197295). Also able to demethylate m6A in U6 small nuclear RNA (snRNA) (PubMed:30197295). Mediates demethylation of N6,2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N6-methyladenosine at the second transcribed position of mRNAs and U6 snRNA (PubMed:28002401, PubMed:30197295).
Verified picture:
Immunohistochemistry of paraffin-embedded Human breast cancer using FTO antibody diluted at 1:100
Western blot analysis with FTO antibody diluted at 1:2000;Lane:CoLO205,Jurkat,U251,THP-1,A549
Immunohistochemistry of paraffin-embedded Human testis cancer using FTO antibody diluted at 1:100