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Anti-PAM Polyclonal Antibody
Cat: K110535P
Summary:
【Product name】:Anti-PAM Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG 【Purification】:Affinity purification
【Gene ID】:5066 【Swiss Prot】:P19021
【Tested applications】:IHC
【Recommended dilution】:IHC 1:100-300.
【Immunogen】:Recombinant protein of human PAM
【Public Immunogen Range】:1-350aa
【Storage】:Store at -20℃. Avoid freeze/thaw cycles.
Background:
Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate.
Verified picture:
Immunohistochemistry of paraffin-embedded Human pancreatic cancer using PAM antibody diluted at 1:200