Anti-MLKL Monoclonal Antibody
Cat: K009943M
Summary:
| 【Product name】：Anti-MLKL Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Protein A purification |
| 【Gene ID】：197259 | 【Swiss Prot】：Q8NB16 |
| 【Tested applications】：ELISA,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:5000-50000. IHC 1:500-2000. IF 1:50-500. | |
| 【Immunogen】：Recombinant protein of human MLKL. | |
| 【Public Immunogen Range】：1-263aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291)
Verified picture:
Western blot analysis with MLKL antibody diluted at 1:10000;Lane: HT-29 PC-3 HeLa HepG2 NIH/3T3.
Immunohistochemistry of paraffin-embedded human liver cancer with MLKL antibody diluted at 1:1000.
Immunofluorescence analysis of human liver cancer using MLKL antibody diluted at 1:100.