Anti-ACTR3 Polyclonal Antibody
Cat: K111183P
Summary:
| 【Product name】：Anti-ACTR3 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：10096 | 【Swiss Prot】：P61158 |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human ACTR3 | |
| 【Public Immunogen Range】：1-350aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.
Verified picture:
Western blot analysis with ACTR3 antibody diluted at 1:2000;Lane: HL-60,RAW264.7,Mouse spleen.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with ACTR3 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with ACTR3 antibody diluted at 1:100.