Anti-NF-H/NF200 Monoclonal Antibody
Cat: K009971M
Summary:
| 【Product name】：Anti-NF-H/NF200 Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG2a | 【Purification】：Protein A purification |
| 【Gene ID】：4744 | 【Swiss Prot】：P12036 |
| 【Tested applications】：ELISA,FCM,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:5000-50000. IHC 1:4000-16000. IF 1:20-200. | |
| 【Immunogen】：Recombinant protein of human NF200 | |
| 【Public Immunogen Range】：1-214aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.
Verified picture:
Western blot analysis with NF-H antibody diluted at 1:10000;Lane: Pig cerebellum.
Immunohistochemistry of paraffin-embedded rat cerebellum using NF-H antibody diluted at 1:8000.
Immunofluorescence analysis of rat brain using NF-H antibody diluted at 1:400.