Anti-TFB2M Polyclonal Antibody
Cat: K112044P
Summary:
| 【Product name】：Anti-TFB2M Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：64216 | 【Swiss Prot】：Q9H5Q4 |
| 【Tested applications】：IHC,WB | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human TFB2M | |
| 【Public Immunogen Range】：1-285aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA.In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand.Stimulates transcription independently of the methyltransferase activity.
Verified picture:
Western blot analysis with TFB2M antibody diluted at 1:2000;Lane: K562,PC-3M-IE8.
Immunohistochemistry of paraffin-embedded Human Colorectal cancer with TFB2M antibody diluted at 1:100.